The effect of hydrogen peroxide/colloidal silver on reducing the colonization and growth of heterotrophic bacteria in dental unit waterlines

Authors : Meral ÖZALP, Ömer Engin BULUT, Atilla Stephan ATAÇ, Melike EKİZOĞLU, Didem KART, Hakan Hamdi ÇELİK, İlkan TATAR

Pages : 336-341

Doi:10.3906/biy-1204-23

View : 17 | Download : 6

Publication Date : 2013-12-01

Article Type : Research Paper

Abstract :The objective of the present study was to investigate reduction in the colonization and growth of heterotrophic bacteria in dental unit waterlines by using hydrogen peroxide/colloidal silver as disinfectant. Twenty-seven dental units were included; 6 units that were more than 20 years old and 7 units that had been in use for 2 years comprised the old and new treatment groups, respectively. Fourteen units served as controls. The treatment groups were disinfected continuously and every 4 weeks shock doses were applied over a 20-week period. Water samples were taken before treatment, 1 and 2 weeks after treatment, and thereafter every 4 weeks; then they were inoculated onto R2A agar plates. While 1-16-week results for the old treatment group showed total heterotrophic bacterial counts of higher than 1 × 105 cfu/mL, at 20 weeks they were below 7.5 × 102 cfu/mL. Only 2 units were able to reach levels of
Keywords : Key words Biofilm, aerobic heterotrophic bacterial count, dental unit waterlines, hydrogen peroxide colloidal silver