Phylogenetic analysis of Rhizoctonia solani AG-4 isolates from common beans in Black Sea coastal region, Turkey, based on ITS-5.8S rDNA

Authors : Melike Çebi KILIÇOĞLU, İbrahim ÖZKOÇ

Pages : 18-24

Doi:10.3906/biy-1202-17

View : 19 | Download : 9

Publication Date : 2013-12-01

Article Type : Research Paper

Abstract :In this study, 114 Rhizoctonia solani isolates were obtained from diseased common bean insert ignore into journalissuearticles values(Phaseolus vulgaris L.); plants from the Black Sea coastal region of Turkey. Genetic variation was determined among R. solani anastomosis group 4 insert ignore into journalissuearticles values(AG-4); subgroups insert ignore into journalissuearticles values(AG-4 HG-I and AG-4 HG-II);. ITS-5.8 rDNA sequences of these isolates were aligned with other known R. solani sequences from GenBank, and distance and parsimony analysis were used to determine phylogenetic relationships. The R. solani AG-4 isolates were placed in 2 main lineages, corresponding to AG-4 HG-I and AG-4 HG-II. Based on phylogenetic analysis, genetic variations were shown in the AG-4 subgroups insert ignore into journalissuearticles values(especially AG-4 HG-I);. This study represents the first reported use of rDNA-ITS sequence data to examine AG-4 HG-I and HG-II in P. vulgaris from Turkey. The results also suggest that the Giresun isolates are closely related to isolates obtained from Italy.
Keywords : Key words rDNA ITS phylogeny, Rhizoctonia solani, AG 4, Phaseolus vulgaris, common bean