The effect of 3-methylcholanthrene and butylated hydroxytoluene on glycogen levels of liver, muscle, testis, and tumor tissues of rats

Authors : Fikriye POLAT, Egemen DERE, Eylem GÜL, İzzet YELKUVAN, Öztürk ÖZDEMİR, Günsel BİNGÖL

Pages : 33-38

Doi:10.3906/biy-1010-126

View : 23 | Download : 9

Publication Date : 2013-12-01

Article Type : Research Paper

Abstract :This study examined the effects of separate and combined applications of 3-methylcholanthrene, a polycyclic aromatic hydrocarbon and potent carcinogenic agent, and butylated hydroxytoluene, the antioxidant food additive, on the glycogen levels of liver, muscle, testis, and tumor tissues in rats. Adult male Wistar albino rats weighing 100-110 g at 8 weeks of age were used in this study. This study consisted of a control group insert ignore into journalissuearticles values(n = 9); and 3 different experiment groups in which rats were chronically treated with 3-methylcholanthrene insert ignore into journalissuearticles values(n = 9); or butylated hydroxytoluene insert ignore into journalissuearticles values(n = 11); or a combination of these agents insert ignore into journalissuearticles values(n = 14);. Rats were intraperitoneally injected with a 200 mg kg-1 dose of butylated hydroxytoluene and a 40 mg kg-1 dose of 3-methylcholanthrene. At the end of the 26-week experimental period, tissues of rats killed via cervical dislocation were placed in 10% trichloroacetic acid for glycogen determination. Our results showed that the administration of 3-methylcholanthrene, butylated hydroxytoluene, and 3-methylcholanthrene + butylated hydroxytoluene caused statistically significant changes in the glycogen levels of liver, muscle, and testis tissues, and glycogen was stored in tumor tissue.
Keywords : Key words Glycogen, 3 methylcholanthrene, butylated hydroxytoluene, rat