Antioxidant activity of in vitro propagated Stevia rebaudiana Bertoni plants of different origins

Authors : Ely ZAYOVA, Ira STANCHEVA, Maria GENEVA, Maria PETROVA, Lyudmila DIMITROVA

Pages : 106-113

Doi:10.3906/biy-1204-64

View : 15 | Download : 9

Publication Date : 2013-12-01

Article Type : Research Paper

Abstract :An efficient in vitro protocol for propagation of Stevia rebaudiana Bertoni is described. Multiple shoots were induced in vitro from shoot tip and nodal segments on Murashige and Skoog medium containing 6-benzylaminopurine, zeatin, or thidiazuron alone and in combination with naphthalene acetic acid or indole-3-acetic acid. A high frequency of shoot induction as well as maximum number of shoots per shoot tip explant was observed on Murashige and Skoog medium supplemented with 6-benzylaminopurine insert ignore into journalissuearticles values(1.0 mg L-1); alone and combined with indole-3-acetic acid insert ignore into journalissuearticles values(0.1 mg L-1);. For root induction, in vitro shoots were transferred to rooting media containing naphthalene acetic acid, indole-3-acetic acid, or indole-3-butyric acid. The highest rooting frequency and the highest number of roots was observed in half-strength Murashige and Skoog medium supplemented with 0.1 mg L-1 indole-3-butyric acid. The rooted in vitro plants were successfully acclimatized in a growth chamber and transferred to the field. Leaf extracts of plants propagated in vitro and adapted to field conditions are characterized by high levels of water-soluble antioxidant capacity insert ignore into journalissuearticles values(expressed as equivalents of ascorbic acid);, phenols, and flavonoids, and therefore by high total antioxidant potential, expressed as DPPH radical scavenging activity.
Keywords : Key words Acclimatization, micropropagation, nodal segments, shoot tips