Effects of α-lipoic acid on DNA damage, protein oxidation, lipid peroxidation, and some biochemical parameters in sub-chronic thinner-addicted rats

Authors : Muhsin KONUK, Tuğba ŞAHİN, İbrahim Hakkı CİĞERCİ

Pages : 702-710

View : 24 | Download : 13

Publication Date : 2012-12-01

Article Type : Research Paper

Abstract :The present study was carried out to determine the effects of sub-chronic thinner addiction on the oxidant-antioxidant balance, the relation between toxicity and oxidative stress, and a possible protective effect of a-lipoic acid against thinner toxication in rats. Sprague-Dawley rats were divided into 5 groups as follows: control insert ignore into journalissuearticles values(K);, olive oil insert ignore into journalissuearticles values(Z);, a-lipoic acid insert ignore into journalissuearticles values(L);, thinner insert ignore into journalissuearticles values(T);, and a-lipoic acid + thinner insert ignore into journalissuearticles values(LAT);. Each group was composed of 15 rats, and the study lasted 8 weeks. After completing the animal studies malondialdehyde insert ignore into journalissuearticles values(MDA);, reduced glutathione insert ignore into journalissuearticles values(GSH);, methemoglobin insert ignore into journalissuearticles values(MetHb);, toluol, and mononuclear leucocyte damage levels; protein oxidation, nitric oxide insert ignore into journalissuearticles values(NOx); metabolites, total antioxidant capacity insert ignore into journalissuearticles values(TAC);, glucose, total cholesterol, triglycerides, HDL-cholesterol, LDL-cholesterol, aspartate aminotransferase insert ignore into journalissuearticles values(AST);, and alanine aminotransferase insert ignore into journalissuearticles values(ALT); levels were determined from blood specimens of the rats. The data obtained from the study were statistically analyzed using SPSS, and both ANOVA and Duncan tests were performed. P < 0.05 was accepted as statistically significant. The results indicated that a-lipoic acid has a protective and balancing effect against complications derived from thinner inhalation in rats.
Keywords : Key words Thinner, oxidative stress, a lipoic acid, thinner inhalation