DATS suppresses invasion of oral squamous cell carcinoma cell lines by reducing matrix metalloproteinase-9 via PI3K/AKT

Authors : Jianbin YANG, Dongyi WEI, Zhiyan WU, Suhua XU

Pages : 506-512

View : 21 | Download : 10

Publication Date : 2012-12-01

Article Type : Research Paper

Abstract :Oral squamous cell carcinomas insert ignore into journalissuearticles values(OSCCs); are the most common type of oral cancers, and high morbidity and mortality are largely attributable to late-stage diagnosis. Despite significant advances in therapeutic strategies, the 5-year survival rate still remains low. Diallyl trisulfide insert ignore into journalissuearticles values(DATS);, an oil-soluble organosulfur compound of garlic, was recently reported for its anticancer effect on several types of cancer cells in vitro; however, these anticancer effects are still unknown in human OSCC cells. In the present study, the effects of DATS on OSCC cell invasion in vitro were investigated. Human OSCC cell lines insert ignore into journalissuearticles values(HSC-2 and Ca9-22 cells); were treated with DATS and examined for cell viability by 3-insert ignore into journalissuearticles values(4,5-dimethylthiazol-2-yl);-2,5-diphenyltetrazolium bromide insert ignore into journalissuearticles values(MTT); assay; the suppression of cell invasion was demonstrated by transwell assay. Western blot was performed to detect the effect of DATS on the expression of matrix metalloproteinase-9 insert ignore into journalissuearticles values(MMP-9); and the activation of v-akt murine thymoma viral oncogene homolog 1 insert ignore into journalissuearticles values(AKT1);, respectively. The results showed that DATS insert ignore into journalissuearticles values(at 40 mM, a nontoxic dose); and GM6001 inhibited insert ignore into journalissuearticles values(P < 0.05); the invasion of OSCC cells, respectively, when compared to the DMSO control. DATS insert ignore into journalissuearticles values(at 40 mM); significantly decreased insert ignore into journalissuearticles values(P < 0.05); the expression of MMP-9 and time-dependently inhibited insert ignore into journalissuearticles values(P < 0.05); the activation of AKT1, respectively. Moreover, LY294002 also decreased insert ignore into journalissuearticles values(P < 0.05); the expression of MMP-9 in a dose-dependent manner. It was concluded that DATS inhibits the invasion of OSCC cells by decreasing MMP-9 expression via the PI3K/AKT pathway.
Keywords : Key words DATS, oral squamous cell carcinomas, invasion, MMP 9, AKT