Cardioprotective effect of diazepam on ischemia-reperfused isolated hyperthyroid rat heart

Authors : Dareuosh SHACKEBAEI, Atefeh ASADMOBINI, Mahvash HESARI, Maryam VAEZI, Siamak SHAHIDI

Pages : 598-605

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Publication Date : 2012-12-01

Article Type : Research Paper

Abstract :Hyperthyroidism increases the vulnerability of the heart to ischemia-reperfusion insert ignore into journalissuearticles values(I/R); injury. Regarding the fact that diazepam also affects cardiac I/R injury, the current study was conducted to investigate the effect of diazepam on ischemia-reperfused isolated hyperthyroid rat heart. The animals were divided into 6 groups: the control, hyperthyroid, control diazepam insert ignore into journalissuearticles values(1 mg/kg);, hyperthyroid diazepam insert ignore into journalissuearticles values(1 mg/kg);, control diazepam insert ignore into journalissuearticles values(5 mg/kg);, and hyperthyroid diazepam insert ignore into journalissuearticles values(5 mg/kg); groups. Langendorff-perfused rat hearts underwent 40 min of global ischemia and 45 min of reperfusion. Different cardiac parameters, including the left ventricular developed pressure, heart rate, coronary flow, and rate pressure product insert ignore into journalissuearticles values(RPP);, were measured. Lactate dehydrogenase insert ignore into journalissuearticles values(LDH); release was determined in reperfusion to evaluate the severity of the myocardial injury. The results showed that the RPP recovery percentage significantly decreased in the hyperthyroid group compared with control group, whereas comparison of the hyperthyroid diazepam insert ignore into journalissuearticles values(1 mg/kg); with the control and control diazepam insert ignore into journalissuearticles values(1 mg/kg); groups did not reveal a significant difference. These results were confirmed by LDH test, demonstrating that administration of diazepam insert ignore into journalissuearticles values(1 mg/kg); protected the heart against I/R injury in the hyperthyroid group and significantly decreased the I/R injury, which was probably due to the function of diazepam as a phosphodiesterase 4 inhibitor.
Keywords : Key words Diazepam, hyperthyroidism, isolated heart, ischemia reperfusion, cardioprotective