Cloning and sequencing of coat protein gene of Zucchini yellow mosaic virus isolated from squash and muskmelon in Turkey

Authors : Meryem ÖZER, Hikmet Murat SİPAHİOĞLU, Mustafa USTA, Hakan FİDAN

Pages : 423-429

View : 19 | Download : 6

Publication Date : 2012-12-01

Article Type : Research Paper

Abstract :The coat protein insert ignore into journalissuearticles values(CP); genes of the genomic RNA of 2 severe Turkish isolates of Zucchini yellow mosaic virus insert ignore into journalissuearticles values(ZYMV); from squash and muskmelon [ZYMV-Adana insert ignore into journalissuearticles values(Ad); and ZYMV-Ahlat insert ignore into journalissuearticles values(Ah);, respectively] were cloned, and their complete nucleotide sequences and deduced amino acids were determined. The analysis revealed that both Turkish ZYMV-CP genes contained 837 nucleotides encoded for a CP of about 31.2 kDa. Phylogenetic trees based on nucleic acid sequences were constructed by the neighbor joining and unweighted pair group mean arithmetic insert ignore into journalissuearticles values(UPGMA); methods with 100 bootstrap replicates. A high degree of homology was detected between the 2 Turkish isolates on the nucleotide sequences insert ignore into journalissuearticles values(97%);. The CP sequence of ZYMV-Ad and ZYMV-Ah varied among the 23 isolates with overall identity of 93%-98% and 94%-99%, respectively, at the nucleotide level. Comparison of the nucleotide sequences of 23 isolates from different geographical regions worldwide showed the ZYMV-Ad isolate clustered with isolates from Middle Eastern countries insert ignore into journalissuearticles values(Israel, Jordan, and Syria);; ZYMV-Ah isolate was clustered with isolates from Far Eastern countries insert ignore into journalissuearticles values(Korea and Taiwan);. The N-terminal of the Turkish ZYMV-Ah CP contained a distinctive sequence at nucleotide positions 9324-9328, which distinguished the Turkish ZYMV-Ah isolate from all previously reported ZYMV isolates. The CP cistron of the ZYMV-Ad and Ah isolates contained 279 amino acid residues. Pairwise nucleotide sequence comparison revealed sequence similarities of 58%-70% between ZYMV Turkish isolates and 22 other potyviruses. Mechanical inoculations showed that ZYMV-Ah produced faster systemic symptom induction than ZYMV-Ad on squash, suggesting that ZYMV-Ah was a more severe isolate than ZYMV-Ad insert ignore into journalissuearticles values(GenBank accession JF317296-JF317297);.
Keywords : Key words ZYMV, characterization, coat protein, phylogenetic analysis