Antioxidant enzymes and GST activity in natural populations of Holandriana holandrii from the Bosna River

Authors : Jelena VRANKOVIC, Svetlana LABUSBLAGOJEVIC, Bela CSANYI, Jarmila MAKOVINSKA

Pages : 477-485

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Publication Date : 2012-12-01

Article Type : Research Paper

Abstract :Specimens of the freshwater snail Holandriana holandrii affected by different levels of contamination were collected from 3 sites within the Bosna River Basin, i.e. Visoko, Doboj, and Modrica. The activities of antioxidant enzymes such as superoxide dismutase insert ignore into journalissuearticles values(SOD);, catalase insert ignore into journalissuearticles values(CAT);, glutathione peroxidase insert ignore into journalissuearticles values(GPx);, glutathione reductase insert ignore into journalissuearticles values(GR);, and glutathione-S-transferase insert ignore into journalissuearticles values(GST); were investigated in order to understand their variation with respect to the pollution status of the sampling locations. The results revealed a significant increase in CAT, GPx, and GST activities in the snails collected from Modrica, suggesting that the animals at this location are exposed to a higher level of oxidative stress as compared to those from Visoko and Doboj. On the other hand, increased SOD activity measured in specimens from Visoko was indicative of the presence of increased levels of superoxide anion radical. No snails from any location were significantly exposed to organic pollution, since its concentration in the whole body homogenates was below the limit of detection. Our findings show that changes in antioxidant enzymes and GST activity can be used as parameters in environmental monitoring programs.
Keywords : Key words Antioxidant enzyme, GST, freshwater snail, biomarker, bioindicator