Genetic variability of Beauveria bassiana and Metarhizium anisopliae var. anisopliae isolates obtained from the Eastern Black Sea Region of Turkey

Authors : Ali SEVİM, Monica HÖFTE, Zihni DEMİRBAĞ

Pages : 255-265

View : 14 | Download : 7

Publication Date : 2012-12-01

Article Type : Research Paper

Abstract :Beauveria bassiana insert ignore into journalissuearticles values(Balsamo); Vuillemin and Metarhizium anisopliae var. anisopliae insert ignore into journalissuearticles values(Metschnikoff); Sorokin are the most common entomopathogenic fungi used in microbial control programs all over the world. Assessments of the genetic variability of these 2 important species are useful for the development of effective biocontrol strategies and for evaluating the impact of artificial epizootics. In this study, the genetic diversity of 13 B. bassiana and 33 M. anisopliae var. anisopliae strains isolated from the hazelnut-growing region of Turkey was determined by using the amplified fragment length polymorphism insert ignore into journalissuearticles values(AFLP); and a- and b-isoenzyme analyses. Cluster analysis of AFLP data clearly separated both B. bassiana and M. anisopliae var. anisopliae strains into 3 and 4 different groups, respectively. While a- and b-esterase banding patterns clearly separated M. anisopliae var. anisopliae strains, it did not give enough information about B. bassiana strains. We also assessed the growing ability of all isolates at different temperatures insert ignore into journalissuearticles values(8, 16, 25, and 37 °C); and UV exposures insert ignore into journalissuearticles values(30 and 60 min);, and virulence against Tenebrio molitor. These results indicated that there is a significant variability within the B. bassiana and M. anisopliae var. anisopliae populations in this region. Although the diversity of B. bassiana isolates is associated with geographic location, it is not associated with habitat type. There is also no association amongst B. bassiana isolates in terms of the ability to grow at different temperatures and UV exposures, or virulence against T. molitor. The diversity of M. anisopliae var. anisopliae strains is neither associated with habitat type nor geographic location, and there is no association amongst M. anisopliae var. anisopliae strains in terms of the ability to grow at different temperatures insert ignore into journalissuearticles values(except for 16 °C); and UV exposure, or virulence against T. molitor. The data presented here might be useful for controlling some hazelnut pests in this region.
Keywords : Key words Beauveria bassiana, Metarhizium anisopliae, genetic variability, hazelnut, geographic location