Phylogenetic characterization, antimicrobial susceptibilities, and mechanisms of resistance in bacteria isolates from a poultry waste-polluted river, southwestern Nigeria

Authors : Olawale Olufemi ADELOWO, Obasola Ezekiel FAGADE

Pages : 37-45

View : 17 | Download : 8

Publication Date : 2012-12-01

Article Type : Research Paper

Abstract :Bacteria resistance to antibiotics has become a worldwide problem impacted partly by antibiotics use in livestock production. The present study investigated the pattern and mechanisms of resistance in 21 bacteria species isolated from a river used as a waste-dump by a poultry farm in Ogbomoso, southwestern Nigeria. Bacteria were isolated on nutrient agar supplemented with 50 µg/mL of tetracycline, their identities were determined by PCR amplification, and sequencing of their 16s rRNA genes and their susceptibility to 6 antimicrobials determined by broth dilution. The molecular basis of resistance among the isolates was also investigated by the PCR amplification of class 1 and 2 integrons, tetA, tetB, tetC, tetD, tetE, tetG, tetL, tetW, tetM, tet37, blaTEM, aadA1, strA, strB, sul2, sul3 and a 550bp gene fragment encoding a multidrug resistance efflux transporter of the resistance nodulation division insert ignore into journalissuearticles values(RND); family. All the isolates were resistant to the tested antimicrobials with MIC ranging from
Keywords : Key words 16s rRNA, resistance genes, RND transporter, integrons