Antimicrobial activity of poplar propolis on mutans streptococci and caries development in rats

Authors : Soley ARSLAN, Sibel SİLİCİ, Duygu PERÇİN, Ayşe Nedret KOÇ, Özgür ER

Pages : 65-73

View : 25 | Download : 11

Publication Date : 2012-12-01

Article Type : Research Paper

Abstract :The influence of the different extracts of propolis and chlorhexidine on mutans streptococci and the development of dental caries in rats was examined in this study. Specifically, the antimicrobial effects of propolis extracts insert ignore into journalissuearticles values(including ethanol, methanol, chloroform, hexane, propylene glycol, and ethyl acetate); on Streptococcus mutans UA159 and Streptococcus sobrinus 6715 were studied. Minimal inhibitory concentration and minimal bactericidal concentration were determined using the National Committee for Clinical Laboratory Standards broth macrodilution method. In the animal study, the rats were infected with S. sobrinus and fed a cariogenic diet. They were treated topically twice a day with both ethanol extract of propolis insert ignore into journalissuearticles values(EEP); and hexane extract of propolis insert ignore into journalissuearticles values(HEP); for 5 weeks. After the experimental period, both the microbial composition of their dental plaque and their caries score were assessed. EEP and HEP showed the highest antimicrobial activity against the tested microorganisms. The chemical composition of EEP and HEP were examined using gas chromatography-mass spectrometry. The pinobanksin and naringenin were the main phenolic compounds identified in both extracts. Further, both of the extracts significantly reduced the severity of smooth surface caries in vivo. The severity of the sulcal caries insert ignore into journalissuearticles values(Ds); was significantly reduced by EEP compared to the control group insert ignore into journalissuearticles values(P < 0.05);. In conclusion, poplar propolis demonstrated antimicrobial activity against mutans streptococci, indicating that it may be used in caries prevention.
Keywords : Key words Antimicrobial activity, dental caries, propolis, S mutans, S sobrinus