Studies on the effect of pH and carbon sources on enzyme activities of some pectinolytic bacteria isolated from jute retting water

Authors : Biswapriya DAS, Ashis CHAKRABORTY, Sagarmoy GHOSH

Pages : 671-678

View : 17 | Download : 8

Publication Date : 2011-12-01

Article Type : Research Paper

Abstract :The effect of pH of the assay media and different carbon sources in the culture media on the activities of 4 different enzymes, namely polygalacturonase insert ignore into journalissuearticles values(PG);, pectin lyase insert ignore into journalissuearticles values(PNL);, xylanase, and cellulase, of some pectinolytic bacteria were studied. The pectinolytic bacterial strains were isolated from the jute retting water bodies of 2 important jute growing areas of North 24 Parganas, West Bengal, India. The highest PG insert ignore into journalissuearticles values(12.45 to 41.0 IU/g cell wet weight); and PNL insert ignore into journalissuearticles values(22.55 to 75.7 U/mL); activities occurred at the alkaline pH of 8.0 and 8.5, respectively. Maximum xylanase activity insert ignore into journalissuearticles values(0.01 to 0.735 mmol/mL/min); occurred at pH 7.0. An acidic pH of 5.0 favored the cellulase activity insert ignore into journalissuearticles values(0 to 0.168 mmol/mL/min);. All the enzymes were highly substrate specific and inducible in nature. The highest PG and PNL activities were observed in the presence of pectin, whereas the highest xylanase and cellulase activities were detected in the presence of xylan and cellulose, respectively, as the sole carbon source in the media.
Keywords : Key words Pectinolytic bacteria, polygalacturonase, pectin lyase, xylanase, cellulase