Production of a novel bifunctional catalase-phenol oxidase of Scytalidium thermophilum in the presence of phenolic compounds

Authors : Yonca YÜZÜGÜLLÜ, Zümrüt Begüm ÖGEL, Ufuk Bakir BÖLÜKBAŞI, Nursen ÇORUH, Gürkan KARAKAŞ

Pages : 697-704

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Publication Date : 2011-12-01

Article Type : Research Paper

Abstract :A novel bifunctional catalase with additional phenol oxidase activity insert ignore into journalissuearticles values(CATPO); is produced by the thermophilic fungus, Scytalidium thermophilum, in a growth-associated manner. In order to study the biological significance of this dual enzyme in relation to phenolic compounds, 14 phenolics were tested for their effect on growth and CATPO production. It was determined that some phenolics exerted a negative effect on growth insert ignore into journalissuearticles values(catechol, coumaric acid, hydroquinone, kaempferol, myricetin);, while others either did not influence growth in a negative manner or enhanced growth by a maximum of 50% increase in biomass insert ignore into journalissuearticles values(caffeic acid, chlorogenic acid, catechin, gallic acid, resorcinol, vanillic acid);. Hydroquinone and myricetin showed an antifungal effect and enhanced CATPO production, while catechol, coumaric acid, and kaempferol decreased CATPO production and showed a toxic effect at higher doses. In general, phenolics acting in an antioxidant manner exhibited a reverse interrelation with CATPO production. These results suggest that the presence of antioxidant phenolic compounds has an effect on enhancing its antioxidant activity; enzyme is therefore no longer required.
Keywords : Key words Antioxidant, catalase, phenol oxidase, phenolic compound, Scytalidium thermophilum, thermophilic fungus