A comparative study on alkaline lipase production by a newly isolated Aspergillus fumigatus MTCC 9657 in submerged and solid-state fermentation using economically and industrially feasible substrate

Authors : Akhila RAJAN, A Jayakumaran NAIR

Pages : 569-574

View : 15 | Download : 10

Publication Date : 2011-12-01

Article Type : Research Paper

Abstract :A comparative study of solid-state fermentation insert ignore into journalissuearticles values(SSF); and submerged fermentation insert ignore into journalissuearticles values(SmF); for the production of alkaline lipase from a new isolate, Aspergillus fumigatus MTCC 9657, using defatted rice bran insert ignore into journalissuearticles values(DFRB); as a substrate was studied. Different process parameters, such as incubation period, initial pH and incubation temperature, were optimised to achieve the maximum yield of the alkaline lipase. Maximum enzyme production insert ignore into journalissuearticles values(8.13 U/mL); was obtained by day 7 of incubation in SSF compared to day 4 in SmF at an optimum pH of 8.5 and ambient temperature of 30 °C. Lipase produced by SSF was stable over a period of 15 days, whereas lipase production in SmF decreased by day 5. Lipase production in SSF with DFRB using sterile water as a moisture source exhibited more activity insert ignore into journalissuearticles values(577.5 U/70 mL); than that supplemented with mineral medium. Compared to the cost of culture medium, the solid-state substrate DFRB is inexpensive, and therefore this process is industrially and economically feasible. These results confirm the interesting potential of SSF in DFRB without any additional nutritional supplement.
Keywords : Key words Defatted rice bran, Aspergillus fumigatus MTCC 9657, alkaline lipase, SSF