The application of ISSR-PCR to determine the genetic relationship and genetic diversity between narrow leaved bluegrass (Poa angustifolia) and rough bluegrass (Poa trivialis) accessions

Authors : Emine ARSLAN, Ahmet TAMKOÇ

Pages : 415-423

View : 25 | Download : 9

Publication Date : 2011-12-01

Article Type : Research Paper

Abstract :: The present study aimed to determine the genetic relationship between Poa angustifolia and Poa trivialis as well as intraspecies genetic diversity using inter simple sequence repeat insert ignore into journalissuearticles values(ISSR); markers. ISSR amplification was evaluated for its applicability to narrow leaved bluegrass and rough bluegrass accessions identification. A total of 401 bands, 363 of which were polymorphic insert ignore into journalissuearticles values(90.52%);, were generated using selected 20 primers. Genetic similarity between accessions belonging to Poa species was estimated using Nei`s genetic similarity and UPGMA insert ignore into journalissuearticles values(unweighted pair group method with arithmetic); cluster analysis was used to construct a dendrogram. UBC836 and M6 primers were observed to be the most suitable for distinguishing between species. Because interspecies differences are especially important in plant breeding, M5 and UBC812 primers were ideal in distinction of P. trivialis and P. angustifolia accessions, respectively. Primer UBC856 was determined to be ideal in discrimination both intraspecies and interspecies. The ISSR fingerprinting technique used was confirmed to be a reproducible and sensitive tool for the identification of Poa accessions and for determination of the correct starting material for plant breeding.
Keywords : Key words Poa angustifolia, Poa trivialis, intersimple sequence repeat ISSR, genetic relationship, genetic diversity