The protective effect of boric acid on aluminum-induced hepatotoxicity and genotoxicity in rats

Authors : Hasan TÜRKEZ, Fatime GEYİKOĞLU, Suat ÇOLAK

Pages : 293-301

View : 21 | Download : 10

Publication Date : 2011-12-01

Article Type : Research Paper

Abstract :The efficacy of boric acid insert ignore into journalissuearticles values(BA); was examined on liver marker enzymes in aluminum insert ignore into journalissuearticles values(Al);-treated rats. Also, a liver micronucleus assay was performed to evaluate the genotoxicity in hepatocytes. With these aims, Sprague-Dawley rats were randomly separated into 10 groups of 5 animals. Aluminum chloride insert ignore into journalissuearticles values(5 mg/kg body weight insert ignore into journalissuearticles values(b.w.); AlCl3); and BA insert ignore into journalissuearticles values(3.25, 13, 36 and 58.5 mg/kg b.w.); alone were administered with injections to the experimental animals. Furthermore, the animals were also treated with Al for 4 consecutive days followed by BA exposure for 10 days. The rats were anesthetized after Al and BA injections and the levels of serum enzymes were determined. Hepatocytes were isolated for counting the number of micronucleated hepatocytes insert ignore into journalissuearticles values(MNHEPs);. After exposure to Al, the enzymatic activities of alkaline phosphatase insert ignore into journalissuearticles values(ALP);, aspartate aminotransferase insert ignore into journalissuearticles values(AST);, alanine aminotransferase insert ignore into journalissuearticles values(ALT);, and lactate dehydrogenase insert ignore into journalissuearticles values(LDH); significantly increased. Furthermore, this metal caused a significant increase in MNHEPs` incidence. In contrast, the applications of BA doses did not cause any adverse effect on the above parameters. Moreover, pretreatments with BA significantly modulated the toxic effects of Al.
Keywords : Key words Aluminum, boric acid, liver, micronucleus assay, rat, serum enzymes