Electrophoretic analysis of genetic diversity in natural house fly (Musca domestica L.) populations from the western and southern coasts of Turkey

Authors : Belgin Göçmen TAŞKIN, Vatan TAŞKIN, Köksal KÜÇÜKAKYÜZ

Pages : 337-346

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Publication Date : 2011-12-01

Article Type : Research Paper

Abstract :In this study genetic variation was determined with isozyme analysis in 8 natural house fly insert ignore into journalissuearticles values(Musca domestica L.); populations from the western and southern coasts of Turkey, a part of the southern Palearctic region. For this purpose, 7 isozyme systems insert ignore into journalissuearticles values(ACP, AOX, CAT, HBDH, HXK, MDH, and SOD); were resolved using the native polyacrylamide gel electrophoresis insert ignore into journalissuearticles values(native-PAGE); technique. A total of 9 loci and 20 alleles were detected. The proportion of polymorphic loci in populations ranged from 44.44% to 66.67%. The overall mean number of effective alleles per locus insert ignore into journalissuearticles values(Ne); was 1.314 ± 0.454. The mean estimated expected heterozygosity insert ignore into journalissuearticles values(He); of populations was 0.177 ± 0.228. A very high proportion insert ignore into journalissuearticles values(93.70%); of genetic diversity was due to variation within populations while a relatively small portion insert ignore into journalissuearticles values(6.30%); was due to variation among populations. The average level of gene flow insert ignore into journalissuearticles values(Nm); was calculated to be 3.7182 per generation among all populations studied. The gene flow detected among the Aegean populations insert ignore into journalissuearticles values(Nm = 6.4451); was 2 times higher than the gene flow detected among the Mediterranean populations insert ignore into journalissuearticles values(Nm = 3.3331);. Our results confirmed the high levels of genetic diversity in house flies reported earlier, supporting the notion that the southern Palearctic region, particularly the Middle East, is the most likely location for the origin of M. domestica.
Keywords : Key words Musca domestica, isozymes, genetic variation, Palearctic region, native PAGE