Determination of antimicrobial and antiproliferative activities of the aquatic moss Fontinalis antipyretica Hedw.

Authors : Filiz SAVAROĞLU, Cansu Filik İŞÇEN, Ayşe Pınar Öztopcu VATAN, Selda KABADERE, Semra İLHAN, Ruhi UYAR

Pages : 361-369

View : 24 | Download : 7

Publication Date : 2011-12-01

Article Type : Research Paper

Abstract :The purpose of the current study was to investigate the possible antimicrobial and anticancer properties of the aquatic moss Fontinalis antipyretica Hedw. insert ignore into journalissuearticles values(Fontinalaceae);. We first obtained 8 different extracts insert ignore into journalissuearticles values(methanol; chloroform; acetone; ethyl acetate A, B, C, and D); by two different extraction processes. The antimicrobial activity of these extracts was then assessed by using the well diffusion method against 8 bacterial and 7 fungal strains. The results of antimicrobial studies showed that extracts of chloroform, acetone, and ethyl acetate A and C were active against almost all the species tested. The most effective extract was C, which was then screened through a preparative TLC insert ignore into journalissuearticles values(bioautography); for its active components. Spot 4 of extract C exhibited the highest antimicrobial activity against 5 bacterial strains insert ignore into journalissuearticles values(B. cereus, B. subtilis, E. faecalis, E. aerogenes, and Y. enterocolitica); and 3 fungal strains insert ignore into journalissuearticles values(A. flavus, F. solani, and F. graminearum);, with MIC values of 93.8-375.0 µg/mL and 187.5-375.0 µg/mL, respectively. In addition, in vitro toxicity of the active component of extract C at the concentrations of 0.16, 1.6, 16, 80, and 160 µg/mL was tested. Depending on dose and time, only 80 and 160 µg/mL decreased rat glioma insert ignore into journalissuearticles values(C6); cell viability after 24 or 48 h. The present study suggests the possibility that Fontinalis antipyretica possesses antimicrobial and anticancer agentinsert ignore into journalissuearticles values(s);.
Keywords : Key words Activity, antibacterial, antifungal, Bryophyta, extract, toxicity