Evaluation of the antioxidant and acetylcholinesterase inhibitory activities of Arnebia densiflora Ledeb.

Authors : İlkay ORHAN, F Sezer ŞENOL, Ufuk KOCA, Tuğba ERÇETİN, Gülnur TOKER

Pages : 111-115

View : 21 | Download : 10

Publication Date : 2011-12-01

Article Type : Research Paper

Abstract :Arnebia densiflora Ledeb. insert ignore into journalissuearticles values(Boraginaceae);, a medicinal plant growing in Turkey, has been reported to contain a well-known red pigment, shikonin, a naphthoquinone derivative. In the current study, the acetylcholinesterase insert ignore into journalissuearticles values(AChE); inhibitory and antioxidant activities of the chloroform, ethyl acetate, methanol, and water extracts of the root, stem, and flowers of the plant, as well as shikonin, were investigated. AChE inhibition was tested using an ELISA microplate reader at 250, 500, 1000, and 2000 µg mL-1. Antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydrazyl insert ignore into journalissuearticles values(DPPH); radical scavenging test and the Fe+2-ferrozine test system for metal chelating power. The results indicated that the methanol and water extracts of the roots had a moderate DPPH radical scavenging effect at 2000 µg mL-1, while the extracts exerted a better ferrous ion chelating effect as compared to the reference, butylated hydroxyanisole. Only the root chloroform extract showed mild inhibition against AChE at 62.5 µg mL-1 insert ignore into journalissuearticles values(49.6 ± 1.69%);. Shikonin was inactive in all of the assays performed.
Keywords : Key words Arnebia densiflora, Boraginaceae, shikonin, antioxidant activity, acetylcholinesterase inhibition