The genotoxic and antigenotoxic effects of Aloe vera leaf extract in vivo and in vitro

Authors : Ahmet KAYRALDIZ, Ayşe Yavuz KOCAMAN, Eyyüp RENCÜZOĞULLARI

Pages : 235-246

View : 19 | Download : 10

Publication Date : 2010-12-01

Article Type : Research Paper

Abstract :The genotoxic and antigenotoxic effects of Aloe vera leaf extract insert ignore into journalissuearticles values(AV); were investigated using the chromosome aberrations insert ignore into journalissuearticles values(CAs); test for the bone marrow cells of rats, sister chromatid exchanges insert ignore into journalissuearticles values(SCEs); and micronucleus insert ignore into journalissuearticles values(MN); and CAs tests for human lymphocytes, and the Ames Salmonella/microsome test system. In the bone marrow cells of rats, AV extract significantly induced structural and total CAs at all concentrations and in all treatment periods. In human peripheral lymphocytes, AV did not increase the mean SCE; however, it significantly induced the MN frequency and structural CAs. In addition, AV showed a cytotoxic effect by decreasing the replication index insert ignore into journalissuearticles values(RI);, mitotic index insert ignore into journalissuearticles values(MI);, and nuclear division index insert ignore into journalissuearticles values(NDI); in human lymphocytes and by decreasing the MI in the bone marrow cells of rats. AV did not decrease the genotoxicity or cytotoxicity of urethane insert ignore into journalissuearticles values(ethyl carbamate, EC); in the bone marrow cells of rats or in the mitomycin-C insert ignore into journalissuearticles values(MMC); in human lymphocytes. AV was a weak mutagen in the TA98 strain of Salmonella typhimurium in the absence of S9mix; however, AV+NPD insert ignore into journalissuearticles values(4-nitro-o-phenylenediamine); and AV+SA insert ignore into journalissuearticles values(sodium azide); exhibited a synergism in increasing the number of revertants for the TA98 and TA100 strains in the absence of S9mix, respectively.
Keywords : Key words Aloe vera, culture of human lymphocytes in vitro, bone marrow cells, rat, Salmonella typhimurium