Propagation of Endangered Thermopsis turcica Tan, Vural & Küçüködük Using Conventional and In Vitro Techniques

Authors : Süleyman CENKCİ, Mehmet TEMEL, Mustafa KARGIOĞLU, Sergun DAYAN

Pages : 327-333

View : 17 | Download : 8

Publication Date : 2009-12-01

Article Type : Research Paper

Abstract :This report deals with the successful clonal propagation of endangered T. turcica using rhizome cuttings and epicotyl explants. Rhizome cuttings were treated with a-naphthaleneacetic acid insert ignore into journalissuearticles values(NAA); or indole-3-butyric acid insert ignore into journalissuearticles values(IBA); before planting for vegetative multiplication. Rhizome cuttings pretreated with NAA insert ignore into journalissuearticles values(10 mg/L); were both rooted and sprouted insert ignore into journalissuearticles values(66.6 percent); after 100 days. Application of NAA induced callus and adventitious root formation in epicotyl explants and 6-benzyladenine insert ignore into journalissuearticles values(BA); induced production of microshoots. Low levels of NAA insert ignore into journalissuearticles values(0.5-1 mM); together with BA promoted shoot initiation and development. The highest regeneration rate insert ignore into journalissuearticles values(86.6 percent);, with a mean number of shoots insert ignore into journalissuearticles values(3.05); and a mean length of shoots insert ignore into journalissuearticles values(2.3 cm); per epicotyl, was achieved at 10 µM BA and 0.5 mM NAA. About 83 percent of in vitro regenerated shoots rooted on a ½ Murashige and Skoog insert ignore into journalissuearticles values(MS); medium supplemented with 0.3 mM NAA. In vitro plantlets were morphologically normal and a uniform chromosome complement of 2n = 18 was detected in root tips. The study demonstrated that both conventional and in vitro techniques could be useful for large scale multiplication and propagation of this endangered plant species.
Keywords : Key words Conservation, rhizome cuttings, epicotyl, propagation, Thermopsis turcica