The toxicity and teratogenicity of gibberellic acid (GA3) based on the frog embryo teratogenesis assay-Xenopus (FETAX)

Authors : Ayper BOĞA, Seçil BİNOKAY, Yaşar SERTDEMİR

Pages : 181-188

View : 17 | Download : 11

Publication Date : 2009-12-01

Article Type : Research Paper

Abstract :This study investigated the developmental toxicity of a plant growth regulator insert ignore into journalissuearticles values(a type of pesticide); using the frog embryo teratogenesis assay-Xenopus insert ignore into journalissuearticles values(FETAX);. Xenopus laevis embryos were exposed to 11 different concentrations of gibberellic acid insert ignore into journalissuearticles values(GA3);, from stage 8 to 11, for 96 h under static renewal test conditions. The median lethal concentration insert ignore into journalissuearticles values(LC50);, malformation insert ignore into journalissuearticles values(EC50);, non-observed adverse effect concentration insert ignore into journalissuearticles values(NOAEC);, and lowest observed adverse effect concentration insert ignore into journalissuearticles values(LOAEC); were calculated. The corresponding LC50 and EC50 values determined for GA3 exposure were 1117.5 mg/L and 658 mg/L, respectively. The TI insert ignore into journalissuearticles values(LC50/EC50); value calculated for GA3 was 1.69. Different anomalies occurred in the embryos, depending on the GA3 concentration. Based on these results, we conclude that GA3 is toxic and teratogenic to Xenopus laevis embryos. Moreover, our results confirm that the FETAX assay can be a useful pretest for integrated biological hazard assessment of chemical agents used in agriculture.
Keywords : Key words Gibberellic acid, FETAX, Teratogenicity, Toxicity, Xenopus laevis