In Vitro Screening of the Antibacterial Activity and Identification of Bioactive Compounds From Plants against Selected Vibrio spp. Pathogens

Authors : Anjana SHARMA, Virendra Kumar PATEL

Pages : 137-144

View : 21 | Download : 10

Publication Date : 2009-12-01

Article Type : Research Paper

Abstract :In vitro investigation of the vibriocidal activity of acetone extracts of Saraca indica Linn insert ignore into journalissuearticles values(Caesalpinaceae); and Datura stramonium Linn insert ignore into journalissuearticles values(Solanaceae);, and aqueous extracts of Allium sativum Linn insert ignore into journalissuearticles values(Liliaceae); showed that they have antibacterial activity against multidrug-resistant human pathogens. Disc diffusion assay showed that there was significant concentration-dependent antibacterial activity against standard strains of Vibrio cholerae, Vibrio parahaemolyticus, and 12 multidrug-resistant isolates of Vibrio cholerae non-O1. The crude plant extracts had zones of inhibition in the range of 7-27 mm against 1 or more test bacteria, with MIC ranging from 2.5 to 15 mg/ml. Acetone extracts of S. indica and D. stramonium, in most cases, had more potency insert ignore into journalissuearticles values(low MIC value); than ethanol and aqueous extracts; however, the aqueous extract of A. sativum was the most effective. Thin layer chromatography insert ignore into journalissuearticles values(TLC);, bioautography, and high performance liquid chromatography insert ignore into journalissuearticles values(HPLC); analysis of the crude extracts revealed that saponin, tannin, scopolamine, atropine, and allicin were the active constituents.
Keywords : Vibriocidal activity, MIC, TLC, TLC bioautography, HPLC