Antibacterial Activity of Aegopodium podagraria L. Extracts and Interaction Between Extracts and Antibiotics

Authors : Olgica STEFANOVIC, Ljiljana COMIC, Dragana STANOJEVIC

Pages : 145-150

View : 15 | Download : 6

Publication Date : 2009-12-01

Article Type : Research Paper

Abstract :Antibacterial activities of aqueous, ethanol, and ethyl acetate extracts of Aegopodium podagraria L. insert ignore into journalissuearticles values(Apiaceae); were tested in vitro against 6 bacteria species. Antibacterial properties were determined by disk diffusion and tube dilution method. Based on the minimal inhibitory concentrations insert ignore into journalissuearticles values(MIC);, the ethanol extract showed the highest activity insert ignore into journalissuearticles values(1.25-5 mg/ml);. Among the tested bacteria, the most sensitive species were Enterobacter cloacae, Klebsiella pneumonia, and Pseudomonas fluorescens, while the most resistant was Staphylococcus aureus. Ethanol extract was chosen to investigate the effects of its combinations with commercial antibiotics by the checkerboard method. Results showed that the interactions between ethanol extract/streptomycin and ethanol extract/chloramphenicol were synergistic, additive, and indifferent against the tested human-pathogenic bacteria. Synergism was observed in relation to Bacillus subtilis. Synergy was confirmed at an ethanol extract concentration corresponding to 1/4 MIC and antibiotic concentrations corresponding to 1/4 MIC and lower.
Keywords : Aegopodium podagraria, antibacterial activity, herb drug interactions, plant extracts