Antioxidant and Antibacterial Activity of Diospyros ebenum Roxb. Leaf Extracts

Authors : Yogesh BARAVALIA, Mital KANERIA, Yogeshkumar VAGHASIYA, Jigna PAREKH, Sumitra CHANDA

Pages : 159-164

View : 13 | Download : 11

Publication Date : 2009-12-01

Article Type : Research Paper

Abstract :The therapeutic properties of plants, including antioxidant and antibacterial activity, have been investigated. In the present study the petroleum ether, ethyl acetate, methanol, and aqueous extracts of Diospyros ebenum Roxb. were evaluated for DPPH insert ignore into journalissuearticles values(2,2-diphenyl-1-picryl-hydrazyl); free radical scavenging insert ignore into journalissuearticles values(antioxidant); activity and antibacterial activity. Antibacterial activity was determined against Bacillus subtilis ATCC6633, Staphylococcus aureus ATCC29737, Pseudomonas aeruginosa ATCC27853, Salmonella typhimurium ATCC23564, and Enterobacter aerogenes ATCC13048 using the agar well diffusion method. Total phenolic and flavonoid content of these extracts was determined as the gallic acid and quercetin equivalent, respectively. Results show that the methanol extract was active against all 5 bacterial strains, whereas the aqueous extract was active against none. The methanol extract had the highest total phenolic content and DPPH free radical scavenging activity insert ignore into journalissuearticles values(IC50 = 20 mg/ml);. The antioxidant and antibacterial potential of the methanol extract of D. ebenum observed in the present study indicates that most active constituents of this plant may be phenolics.
Keywords : Diospyros ebenum, antioxidant activity, antibacterial activity, total phenol content, flavonoid content