Androgenesis in Anther Culture of Lithuanian Spring Barley (Hordeum vulgare L.) and Potato (Solanum tuberosum L.) Cultivars

Authors : Rita ASAKAVICIUTE

Pages : 155-160

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Publication Date : 2008-12-01

Article Type : Research Paper

Abstract :An anther culture method was used for the production of doubled haploids insert ignore into journalissuearticles values(DHs); in Lithuanian barley and potato cultivars that were directly regenerated from embryoids insert ignore into journalissuearticles values(Caredda´s method); were applied to determine androgenic potential according to the green regenerant yield and other morphogenetic factors. Green DH regenerants were obtained in 3 Lithuanian spring barley cultivars insert ignore into journalissuearticles values(´Aidas´, ´Alsa´, and ´Auksiniai´); out of 10 studied. The highest rate of embryoid formation was determined in cv. ´Auksiniai 3´, representing 580.0 embryoids per 100 responding anthers. The potato cultivar ´Nida´ was superior in terms of the rate of responding anthers insert ignore into journalissuearticles values(17.4%);. The highest rate of embryoid formation was identified for the ´Aista´ potato cultivar insert ignore into journalissuearticles values(222.5 embryoids per 100 responding anthers);. The regeneration potential of Lithuanian potato cultivars by direct microspore embryogenesis in the anther culture was evaluated in this experiment. Regenerants were obtained in 3 cultivars insert ignore into journalissuearticles values(´Goda´, ´Nida´, and ´Aista´); out of 5 studied. In the ´Aista´ cultivar, up to 74.5 regenerants per 100 responding anther developed from embryos.
Keywords : Barley, potato, anther culture, responding anthers, embryoids, regenerants, doubled haploids