Antibacterial, Antifungal and Cytotoxic Activities of Tuberous Roots of Amorphophallus campanulatus

Authors : Alam KHAN, Moizur RAHMAN, Shariful ISLAM

Pages : 167-172

View : 19 | Download : 11

Publication Date : 2007-12-01

Article Type : Research Paper

Abstract :Antibacterial, antifungal and cytotoxic activities of ethanol extract of tuberous roots of Amorphophallus campanulatus were studied. Disc diffusion technique was used to determine in vitro antibacterial and antifungal activities. Cytotoxicity was determined against brine shrimp nauplii. In addition, minimum inhibitory concentration insert ignore into journalissuearticles values(MIC); was determined using serial dilution technique to determine antibacterial potency. The extract showed significant antibacterial activities against four gram-positive bacteria insert ignore into journalissuearticles values(Bacillus subtilis, Bacillus megaterium, Staphylococcus aureus, Streptococcus b-haemolyticus); and six gram-negative bacteria insert ignore into journalissuearticles values(Escherichia coli, Shigella dysenteriae, Shigella sonnei, Shigella flexneri, Pseudomonas aeruginosa, Salmonella typhi);. The MIC values against these bacteria ranged from 16 to 128 µg/ml. The antifungal activity was found weak against the tested fungi. In cytotoxicity determination, LC50 of the extract against brine shrimp nauplii was 7.66 µg/ml.
Keywords : Amorphophallus campanulatus, ethanol extract, gram positive, gram negative, MIC, cytotoxicity, antifungal activity