Variation of Callus Induction Through Anther Culture in Water Chestnut (Trapa sp.)

Authors : Aminul HOQUE, Manosh Kumar BISWAS, Shamiul ALAM

Pages : 41-45

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Publication Date : 2007-12-01

Article Type : Research Paper

Abstract :Effects of culture media and genotypes of water chestnut insert ignore into journalissuearticles values(Trapa sp.); on the frequency of callus induction from anther culture were investigated. Results revealed that the N6 media supplemented with 0.5 mg/l BA, 0.5 mg/l NAA and 0.1 mg/l GA3 were suitable for callus induction. Callus yielded poorly in MS-containing media compared to that of N6 media. No callus was induced on 2, 4-D containing media. Among the tested 18 genotypes, only 15 genotypes produced calli.
Keywords : Anther culture, callus, water chestnut