Primary Structure of the Carbohydrate-Binding Modules in Various Cellulolytic, Thermophilic, Anaerobic, Ethanol-Producing Isolates

Authors : Melek ÖZKAN, Gülay ÖZCENGİZ

Pages : 45-50

View : 20 | Download : 10

Publication Date : 2006-12-01

Article Type : Research Paper

Abstract :In the present study, the carbohydrate-binding module insert ignore into journalissuearticles values(CBM); coding sequences of the cellulosomes of 13 thermophilic, cellulolytic, anaerobic, ethanol-producing bacterial isolates having some variations in their growth and cellulose degradation capacities were amplified by PCR and then sequenced. The sequence analysis of the amplicons revealed that CBMs of 7 of the isolates including the isolate 7-9-1 with the highest capacity of cellulose degradation in solid medium have 100% identity in both nucleotide and amino acid sequences to CipB of Clostridium thermocellum in the compared regions. On the other hand, CBM of the isolate 7-1-2, also having a high cellulolytic activity, was found to differ for as much as 66 amino acid residues out of 100 insert ignore into journalissuearticles values(66%);. The isolate 7-9-4 with a relatively low cellulose-degrading capacity also displayed amino acid variation for this protein, but only for 4 out of 118 residues.
Keywords : Cellulose degradation, carbohydrate binding modules, thermophilic and cellulolytic bacteria, Clostridium thermocellum