Anti-Genotoxic Effect of Ascorbic Acid on Mutagenic Dose of Three Alkylating Agents

Authors : Bülent KAYA

Pages : 241-246

View : 20 | Download : 9

Publication Date : 2003-12-01

Article Type : Research Paper

Abstract :The antimutagenic effect of ascorbic acid insert ignore into journalissuearticles values(vitamin C); was investigated by using the Drosophila wing spot test. In this assay, 3-day-old transheterozygous larvae for the multiple wing hair insert ignore into journalissuearticles values(mwh, 3-0.3); and flare insert ignore into journalissuearticles values(flr, 3-38.8); genes were treated with 3 direct acting mutagens: ethyl methanesulfonate insert ignore into journalissuearticles values(EMS);, methyl methanesulfonate insert ignore into journalissuearticles values(MMS); and N-nitroso N-ethylurea insert ignore into journalissuearticles values(ENU);. The results obtained from 3 reference mutagens were clearly genotoxic in the Drosophila wing somatic mutation and recombination test. The calculated concentration of ascorbic acid as recommended dietary allowance insert ignore into journalissuearticles values(RDA); did not induce the frequency of the mutant clones recorded. When co-treatment experiments with ascorbic acid were carried out, ascorbic acid was effective in reducing the genotoxicity of the 3 direct acting mutagens.
Keywords : Drosophila, ascorbic acid, antigenotoxicity, somatic cells