Protein and DNA in Systematic Biology

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Turkish Journal of Biology
Volume:27 Issue:1
Protein and DNA in Systematic Biology

Protein and DNA in Systematic Biology

Authors : Selma GÜLBİTTİ ONARICI, Sibel SÜMER

Pages : 47-55

View : 18 | Download : 8

Publication Date : 2003-12-01

Article Type : Research Paper

Abstract :Since the 1960s, biological macromolecules have occupied an increasingly important role in evolutionary and systematic studies. These biological macromolecules, used as sources of molecular characteristics, are proteins and various types of DNA such as a nuclear genome, nuclear ribosomal DNA, mitochondrial genome, and chloroplast genome. Electrophoresis and immunological techniques are used for generating systematic data from proteins. Generally, electrophoretic methods focus on two general forms of protein data, isozymes and allozymes, while the basis of immunological studies is antigen-antibody reactions. Basic methodologies for using DNA for systematic purposes include DNA-DNA hybridizations, restriction enzyme analysis, sequencing, cloning, PCR and PCR-based fingerprinting techniques.
Keywords : DNA, Protein, Systematic Biology, Phylogeny

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