Effect of Some Biocides on Glutathione-s-transferase in Barley, Wheat, Lentil and Chickpea Plants

Authors : Gülseren COŞKUN, Figen ZİHNİOĞLU

Pages : 89-94

View : 14 | Download : 8

Publication Date : 2002-12-01

Article Type : Research Paper

Abstract :The conjugation of glutathione with herbicides and insecticides is considered to be one of the major detoxification mechanisms of plants. This conjugation is catalysed by a family of multifunctional proteins known as glutathione-s-transferases. Treatment of wheat insert ignore into journalissuearticles values(Triticum aestivum L. cv. Cumhuriyet-75);, barley insert ignore into journalissuearticles values(Hordeum vulgare L. cv. Kaya);, chickpea insert ignore into journalissuearticles values(Cicer arietinum L. cv. Ispanyol);, and lentil insert ignore into journalissuearticles values(Lens culinaris Medik. cv. Kışlık-kırmızı-51); with the selective herbicides 2,4-D insert ignore into journalissuearticles values(2,4-Dichloro phenoxyacetic acid); and linuron insert ignore into journalissuearticles values(N-insert ignore into journalissuearticles values(3,4-dichlorophenyl);-N´-methoxy-N´-methyl urea);, and insecticides parathion methyl insert ignore into journalissuearticles values(O,O-dimethyl O-insert ignore into journalissuearticles values(4-nitrophenyl); phosphorothioate); and malathion insert ignore into journalissuearticles values(S-1,2-bisinsert ignore into journalissuearticles values(ethoxycarbonyl);ethyl O,O-dimethyl phosphorodithioate); caused a progressive increase in glutathione-s-transferase activity in roots and shoots. The maximum increase in enzyme activity was observed in parathion methyl treated wheat roots: 485% of control with a parathion methyl concentration of 1.37 mM. A similar but lower increase in glutathione insert ignore into journalissuearticles values(GSH); levels was observed for roots and shoots, differing according to the origin and the type of pesticide.
Keywords : Glutathione s transferase, detoxification, pesticide