Increasing the Injured Area on Hypocotyl Explants of Flax (Linum usitatissimum L.) Leads to High Frequency Callus-Based Shoot Regeneration

Authors : Mustafa YILDIZ, Celâl ER

Pages : 95-98

View : 17 | Download : 8

Publication Date : 2002-12-01

Article Type : Research Paper

Abstract :In this study, it was aimed to increase the frequency of shoots, which would be nominated to be transgenic after Agrobacterium inoculation, through expanding the wounding area by peeling the epidermis layer of hypocotyl explants of flax plants. Callus weight, percentage of hypocotyls producing shoots, shoot number per explant and total shoot number per petri dish were recorded as 0.8 g, 40%, 4.24 and 16.96, respectively in non-peeled explants. On the other hand, when the epidermis layer was peeled, callus weight, percentage of hypocotyls producing shoots, shoot number per explant and total shoot number per petri dish increased to 1.35 g, 58.75%, 8.15 and 47.88, respectively.
Keywords : Flax, injured area, callus based shoot regeneration