Susceptibility of Different Bean (Phaseolus vulgaris L.) Cultivars to Agrobacterium tumefaciens

Authors : Aziz KARAKAYA

Pages : 447-452

View : 22 | Download : 7

Publication Date : 2001-12-01

Article Type : Research Paper

Abstract :Although several direct gene transfer techniques have been developed, Agrobacterium tumefaciens is still preferred for the transfer of foreign genes into many plant species. Perhaps the greatest weakness of A. tumefaciens is the host range limitation. Therefore, in this study, the susceptibility of different bean cultivars to oncogenic A. tumefaciens strain A281 was assessed. The cultivars 4F-2409, Karacaşehir-90, Akman-98 and Eskişehir-855 resulted in higher frequencies of tumor formation than the other cultivars tested. Tumor formation was not observed in Afyon-3, IVD-10, Göynük-98, Yunus-90 or Şehirali-90 bean cultivars. Tumor formation was observed on both the hypocotyl and epicotyl portions of the plants.
Keywords : Agrobacterium tumefaciens, gene transfer, tumor formation, bean