Availability of Use of Total Extracellular Proteins in SDS-PAGE for Typing Staphylococcus aureus and Coagulase-Negative Staphylococci

Authors : Sefa C SAÇILIK

Pages : 145-151

View : 15 | Download : 12

Publication Date : 2001-12-01

Article Type : Research Paper

Abstract :A total of nineteen Staphylococcus references, nine of which are Staphylococcus aureus and ten coagulase-negative Staphylococci insert ignore into journalissuearticles values(CNS);, were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis insert ignore into journalissuearticles values(SDS-PAGE);. Whole-cell protein insert ignore into journalissuearticles values(WCP); profiles obtained by SDS-PAGE were compared with banding patterns produced by SDS-PAGE of total extracellular proteins insert ignore into journalissuearticles values(TEP);. No report has yet been issued which explains Staphylococcus typing by SDS-PAGE of extracellular proteins. SDS-PAGE of whole-cell extracts did not usefully distinguish different isolates of Staphylococci. Our preliminary results suggest that SDS-PAGE of total extracellular proteins provides additional criteria and characteristic banding patterns for the study of the epidemiology and characterization of Staphylococci. SDS-PAGE of extracellular proteins provided a rapid, reproducible and discriminative method for characterization of Staphylococci which is easy to perform and interpret.
Keywords : Staphylococcus aureus, coagulase negative staphylococci CNS, protein profiles, typing