Transient Eexpression of b-glucuronidase Reporter Gene in Sainfoin (Onobrychis viciifolia Scop.) Cotyledons Via Microprojectile Bombardmen

Authors : Sertaç ÖNDE

Pages : 171-176

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Publication Date : 2001-12-01

Article Type : Research Paper

Abstract :Transient b-glucuronidase reporter gene insert ignore into journalissuearticles values(GUS); expression was visualised in whole cotyledons of sainfoin insert ignore into journalissuearticles values(Onobrychis viciifolia Scop.); after microprojectile bombardment with pBI221.23 DNA. Optimum rupture disk pressure and sample plate distance were determined to be 1350 psi and 6 cm, respectively. Mean gold particle diameter of 1.0 µm and 48 h post-bombardment histochemical GUS assay was found to be superior to 1.6 µm gold particles and 120 h post-bombardment GUS assay. Microscopic analysis revealed the localisation of the gold particles predominantly in the epidermal cell layer, whereas less than 1% of the particles were localised in sub-epidermal cells, as deep as three cell layers. Availability of a regeneration protocol from sainfoin cotyledons and the potential of the microprojectile bombardment recommend future applications for obtaining transgenic sainfoin plants.
Keywords : Onobrychis viciifolia Scop, sainfoin, transient gene expression, biolistic, particle bombardment