Dechlorination of Bleached Kraft Pulp by Laccase Enzyme Produced from Some White-Rot Fungi

Authors : Arzu taşpinar ÜNAL, Nazif KOLANKAYA

Pages : 67-72

View : 18 | Download : 9

Publication Date : 2001-12-01

Article Type : Research Paper

Abstract :The possibility of using crude laccase in the dechlorination of chlorine-based bleached kraft pulp was investigated. Culture supernatants of seven white-rot fungal strains and kraft-pulp samples taken from E1 insert ignore into journalissuearticles values(Alkaline Extraction-1);, E2 insert ignore into journalissuearticles values(Alkaline Extraction-2); and D2 insert ignore into journalissuearticles values(Chlorinedioxide treatment); stages of chlorine-based bleaching processes were used as the laccase source and substrates respectively during the studies. The dechlorination activity of Trametes versicolor was found to be more than that of the other fungi examined. The addition of a laccase inducer, xylidine, into the culture medium of T. Versicolor led to an increase in dechlorination activity. In the experiment performed to find out the role of laccase activity in dechlorination reactions, a considerable reduction in the dissolved oxygen concentration due to laccase-dependent dechlorination activity was observed. Stepwise enzyme treatment was found to be necessary for increasing the yield of dechlorination.
Keywords : Biodechlorination, Kraft pulp, Laccase, Trametes Coriolus, versicolor