Isolation, Identification and Toxigenic Tests of Aeromonas hydrophila from Several Sources in Izmir

Authors : Ataç UZEL, Füsun UÇAR

Pages : 25-32

View : 17 | Download : 8

Publication Date : 2000-12-01

Article Type : Research Paper

Abstract :A total of 43 samples insert ignore into journalissuearticles values(4 sea water, 5 water, 4 river water, 10 oyster and 10 fish samples); from Izmir were examined for the presence of Aeromonas hydrophila. Alkaline peptone Water insert ignore into journalissuearticles values(APW); for the environmental samples and Trypticase Soy Broth with Ampicillin and Toluidine Blue insert ignore into journalissuearticles values(TSBA); for the food samples were used for enrichment of the samples. In isolation, 5 different selective media were used: DNase Agar with Toluidine blue and Ampicillin insert ignore into journalissuearticles values(DNTA);, Aeromonas Agar insert ignore into journalissuearticles values(AA);, Rimler Shotts Agar insert ignore into journalissuearticles values(RSA);, mA Agar and Aeromonas Mediums Base insert ignore into journalissuearticles values(AMB);. Identification of the isolates was performed according to Bergey`s Manual of Determinative Bacteriology insert ignore into journalissuearticles values(1984); and 9 isolates were identified as A. hydrophila. The enterotoxin production of 9 isolates was investigated by the suckling mouse assay and 8 of the 9 isolates were found to be enterotoxigenic.
Keywords : Aeromanas hydrophila, AMB, suckling mouse assay