- Turkish Journal of Biology
- Volume:24 Issue:Ek
- In Vitro Propagation of Thymus sipyleus Boiss. (Lamiaceae)
In Vitro Propagation of Thymus sipyleus Boiss. (Lamiaceae)
Authors : Bengi Baba ERDAĞ
Pages : 81-86
View : 14 | Download : 8
Publication Date : 2000-12-01
Article Type : Research Paper
Abstract :In vitro propagation of Thymus sipyleus Boiss. insert ignore into journalissuearticles values(Lamiaceae);, an endemic from western Anatolia was investigated. Sterilized seeds of the plant were germinated on modified Murashige-Skoog medium insert ignore into journalissuearticles values(mMS); and Heller`s nutrient media. Then, seedlings were transferred to Murashige-Skoog basal medium containing 0.4 mg/l Naphtaleneacetic acid, insert ignore into journalissuearticles values(NAA); and 3 mg/l Benzyladenin insert ignore into journalissuearticles values(BA); and then they formed callus where they were in contact with the medium. Plantlets which regenerated on the same medium formed multiple shoots within 2 weeks and they were rooted after the 4th subculture.Keywords : Thymus sipyleus, callus formation, plant regeneration