Identification of Lactoce Plasmids in Lactococcus lastic subsp. lactis Stains

Authors : Çağla TÜKEL, Mustafa AKÇELİK

Pages : 405-424

View : 14 | Download : 6

Publication Date : 2000-12-01

Article Type : Research Paper

Abstract :Plasmids encoding lactic acid fermentation ability were investigated in 50 strains of L. lactis subsp. lactis. Acriflavine was used for the elimination of lactose fermentation ability insert ignore into journalissuearticles values(Lac + ); from wild type strains. Lactose negative insert ignore into journalissuearticles values(Lac - ); mutants were selected on lactose indicator agar media. Six different lactose plasmids were identified for fifty strains by comparison of plasmid profiles of Lac - and Lac + mutants. The molecular sizes of these lactose plasmids ranged between 26.3 and 38.5 kb.
Keywords : Lactococcus lactis subsp lactis, lactic acid fermentation, plasmid