The Isolation and Characterization of Glucoamylase Enzyme of an Aspergillus niger Natural Isolate

Authors : Gökhan CORAL

Pages : 601-610

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Publication Date : 2000-12-01

Article Type : Research Paper

Abstract :An extracellular glucoamylase enzyme was isolated from Aspergillus niger Z10 natural isolate in a liquid Czapek Dox medium which contained soluble starch insert ignore into journalissuearticles values(2%); as the sole carbon source. The molecular weight of the enzyme was found to be about 100.000 Daltons through Starch-SDS Polyacrylamide Gel Electrophoresis. The optimal pH range of the enzyme was found to be 4.5-5.5 and it was determined that the thermal stability of this enzyme is quite high.
Keywords : Aspergillus niger, Glucoamylase