Obtaining Biosurfactant from Olive Oil Mill Effluent (OOME) Using Pseudomonas sp.

Authors : Uğur SIDAL

Pages : 611-626

View : 13 | Download : 9

Publication Date : 2000-12-01

Article Type : Research Paper

Abstract :Olive oil mill effluent insert ignore into journalissuearticles values(OOME); is one of the major pollutants produced by the agricultural industry in Mediterranean countries. The olive oil mills in these countries generate about 2-3 million tonnes of effluent annually. OOME is a black liquor containing a water-soluble fraction of ripe olives and water that is used in the process of olive oil extraction. This waste has a high mineral content and a chemical oxygen demand of 25.000-100.000 mg/l. depending on the olive oil extraction process used. This study presents a process for biosurfactant as rhamnolipid production by Pseudomonas sp. from olive oil mill effluent insert ignore into journalissuearticles values(OMME);. For this purpose, some biosurfactant-producing strains were assayed and several strains of Pseudomonas sp. were able to grow on OOME as the sole carbon source and accumulate rhamnolipids. Samples of OOME used as a medium of production were diluted and it was only necesarry to add NaNO 3 insert ignore into journalissuearticles values(2.5 g/l); to the medium. The conversion yield was 0.875 g of rhamnolipid per litre of substrate insert ignore into journalissuearticles values(OOME);. The COD of the OOME was reduced by 50-70% in 72 h.
Keywords : Olive Oil Mill Effluent OOME, Pseudomonas sp, Biosurfactant, Rhamnolipid