Simple Production and Purification of Monoclonal Antibodies in Serum-Free Medium

Authors : Aynur BAŞALP

Pages : 189-196

View : 18 | Download : 7

Publication Date : 2000-12-01

Article Type : Research Paper

Abstract :Although serum is a main source of essential nutrients and growth factors, its use creates highly complex and poorly defined media. The use of serum-free media is more efficient than the use of media containing serum especially when the purification and characterization of antibodies are considered. In this study, we developed a serum-free medium system for cultivation of hybrid cells producing monoclonal antibodies specific for Tobacco Mosaic Virus insert ignore into journalissuearticles values(1F5); and 17b-estradiol insert ignore into journalissuearticles values(10F4); insert ignore into journalissuearticles values(15D4);. Cells were cultivated in a medium with 10 % FCS for three days, and subsequently in serum-free medium for two days. The procedure was repeated once again providing monoclonal antibody in the serum-free supernatants comparable in amounts and activity with that in serum containing media. The method enabled purification of antibodies by a single step in high purity.
Keywords : Hybridoma technology, monoclonal antibody, 1F5, 10F4, 15D4, ELISA, serum free medium, gel filtration chromotography