Production of a -Galactosidase using Bacillus stearothermophilusvar. caldiloactis C953

Authors : Production Of A galactosidase USİNG

Pages : 47-54

View : 37 | Download : 7

Publication Date : 1999-12-01

Article Type : Research Paper

Abstract :An intracellular, thermostable a-galactosidase insert ignore into journalissuearticles values(a-D-galactoside galactohydrolase EC 3.2.1.22.); was produced from a strain of Bacillus stearothermophilus var. calidolactis C953. It was found that the presence of NH 4 Cl in the fermentation medium affected microbial growth and that optimum enzyme production occurred with peptone in various sources of nitrogen where NH 4 Cl was used as the main inorganic nitrogen source. After peptone, in decreasing order of enzyme productivity, came tryptone, soybean meal, soya-neutralized peptone and casamino acid. It was determined that, in various carbon sources, raffinose produced the highest a-galactosidase activity, followed by melibiose, D-galactose and molasses, and that in the medium with soybean meal extract the second highest level of activity occurred. Soybean oligosaccharides insert ignore into journalissuearticles values(raffinose-like sugars);, because of their present low commercial value, could be of pratical use in the production of a-galactosidase.
Keywords : a Galactosidase, Microbial enzyme production, Bacillus stearothermophilus