Endogenous heat shock protein GroEL of A. actinomycetemcomitans preferentially targets primary human CD8+ T cells

Authors : Melis KANT, Bünyamin AKGÜL, Ayten NALBANT

Pages : 104-110

Doi:10.3906/biy-1404-64

View : 20 | Download : 10

Publication Date : 2015-12-01

Article Type : Research Paper

Abstract :Apoptosis can be used to manipulate host cells by bacterial products such as bacterial heat shock proteins insert ignore into journalissuearticles values(Hsp);. One of the virulence factors of periodontal pathogen Aggregatibacter actinomycetemcomitans is heat shock protein GroEL insert ignore into journalissuearticles values(AaGroEL);, which has been shown to interact with host cells. AaGroEL insert ignore into journalissuearticles values(Hsp64); also has the potential to modulate immune system cells. In this study we used endogenous AaGroEL protein as an antigen to study bacterial Hsp-induced apoptosis in different immune system cells. Human peripheral blood mononuclear cells and cell lines were cultured with different doses insert ignore into journalissuearticles values(50-1000 ng/mL); of endogenous AaGroEL at various time points. Apoptosis of the cells was measured by Annexin V and 7AAD labeling. Apoptotic cells were analyzed by flow cytometry. Our data suggested that AaGroEL-responding primary CD8+ T cells were more susceptible to apoptosis than CD4+ T cells. Furthermore, the magnitude of apoptosis in the Jurkat T cell line was higher than that in primary CD8+ T cells. There was no statistically significant level of apoptosis in the chronic myeloid leukemia insert ignore into journalissuearticles values(K562); cell line, which belongs to myeloid lineages. Thus, A. actinomycetemcomitans GroEL protein has more potent apoptotic effect on cells that are derived from a lymphoid progenitor.
Keywords : CD4 T cells, CD8 T cells, bacterial heat shock proteins, GroEL, apoptosis