Antioxidant activity of heme oxygenase 1 in Brassica juncea (L.) Czern.(Indian mustard) under salt stress

Authors : KHUSHBU VERMA, SHUBHAM DIXIT, GYAN SINGH SHEKHAWAT, AFROZ ALAM

Pages : 540-549

Doi:10.3906/biy-1501-28

View : 16 | Download : 7

Publication Date : 2015-12-01

Article Type : Research Paper

Abstract :Antioxidant enzymes play a protective role in plants under oxidative stress. The present study explores the role of antioxidant system heme oxygenase1 insert ignore into journalissuearticles values(HO1); in Brassica juncea insert ignore into journalissuearticles values(L.); Czern. in salt stress conditions. B. juncea seedlings were treated with different NaCl concentrations insert ignore into journalissuearticles values(0-200 mM); for 5 days. Salt stress elicited the highest response at 150 mM NaCl concentration; at this level, gene expression of HO1 was also upregulated. Apart from HO1, ascorbate peroxidase insert ignore into journalissuearticles values(APX);, peroxidase insert ignore into journalissuearticles values(POD);, and glutathione reductase insert ignore into journalissuearticles values(GR); also showed their highest activity at 150 mM NaCl concentration. The study shows that HO1 is indispensable in B. juncea for overcoming the oxidative stress caused by salinity. Understanding the intricacy of the defense network, including HO1, against salt stress will be useful for future research into developing salt-tolerant varieties.
Keywords : biotic stress, antioxidant, Brassica juncea, heme oxygenase, salt stress