Use of in vitro propagation of `Obla?inska? sour cherry in rootstock breeding

Authors : DUSICA DORIC, VLADISLAV OGNJANOV, GORAN BARAC, MIRJANA LJUBOJEVIC, ANKICA PRANJIC, KRUNOSLAV DUGALIC, SEZAİ ERCİŞLİ

Pages : 575-581

Doi:10.3906/biy-1412-85

View : 16 | Download : 9

Publication Date : 2015-12-01

Article Type : Research Paper

Abstract :Prunus cerasus L. `Obla?inska? sour cherry germplasm was established in vitrodirectly from in situ plants on different explant collection dates, enabling quick clonal multiplication and introduction to a rootstock breeding program. Rosette initiation of four investigated genotypes was possible from November to April on the medium containing Schenk and Hildebrandt insert ignore into journalissuearticles values(SH); macroelements, Murashige and Skoog insert ignore into journalissuearticles values(MS); microelements, and vitamins supplemented with insert ignore into journalissuearticles values(in mg L?1); 6-benzyladenine insert ignore into journalissuearticles values(BA);, 0.5; indole-3-butyric acid insert ignore into journalissuearticles values(IBA);, 0.01; gibberellic acid insert ignore into journalissuearticles values(GA3);, 0.1; citric acid, 10; and L-ascorbic acid, 10. The lowest percent of contamination was noted in November and December, when dormant buds were used as an explant source, and the highest percentage was found with actively growing shoot tips. The elongation phase of rosettes initiated from dormant buds remained a major obstacle. Survival rates of shoot tips obtained in April were high and subsequent growth more prominent. An increasing index of multiplication from 1.5 to 1.9 was noted on Driver and Kuniyuki walnut medium insert ignore into journalissuearticles values(DKW); with 0.8 mg L?1 BA and 0.01 mg L?1 IBA in the `OV 32? genotype. Rooting percentages of 71.3% and 81.3% were achieved in `OV 17? and `OV 32? genotypes, respectively, on half-strength MS medium with 1 mg L?1 IBA.
Keywords : Dormant period, elongation, explant collection date, Prunus cerasus L, rooting