Effect of sodium nitroprusside on micropropagation and biochemical parameters of CAB-6P and Gisela 6 cherry rootstocks

Authors : VIRGINIA SARROPOULOU, KORTESSA DIMASSITHERIOU, IOANNIS THERIOS

Pages : 595-610

Doi:10.3906/biy-1409-68

View : 13 | Download : 9

Publication Date : 2015-12-01

Article Type : Research Paper

Abstract :The effects of sodium nitroprusside insert ignore into journalissuearticles values(SNP); on micropropagation of CAB-6P and Gisela 6 cherry rootstocks were investigated. Shoot multiplication of CAB-6P was reduced by adding 4.4 $\mu$M 6-benzylaminopurine insert ignore into journalissuearticles values(BA); and SNP. Gisela 6 microcuttings treated with BA + 30 $\mu$M SNP gave the highest average shoot number insert ignore into journalissuearticles values(4.15); and multiplication percentage insert ignore into journalissuearticles values(100%);. In CAB-6P, $\alpha$-naphthaleneacetic acid insert ignore into journalissuearticles values(NAA); + 30 $\mu$M SNP led to the maximum root number insert ignore into journalissuearticles values(13.79); and fresh weight insert ignore into journalissuearticles values(0.336 g);; average root length insert ignore into journalissuearticles values(82 mm); and rooting percentage insert ignore into journalissuearticles values(95.45%); were greatest after adding 40 and 10 $\mu$M SNP + NAA, respectively. In Gisela 6, NAA + 40 $\mu$M SNP resulted in the highest root number insert ignore into journalissuearticles values(9.75); and fresh weight insert ignore into journalissuearticles values(0.238 g);, while NAA + 30 $\mu$M SNP enabled maximum root length insert ignore into journalissuearticles values(30.77 mm);. Rooting percentage was greatest insert ignore into journalissuearticles values(84.62%); when Gisela 6 microcuttings were treated only with NAA. Application of SNP alone in CAB-6P reduced leaf chlorophyll, carbohydrate, and pr?line levels. In contrast, 30?50 $\mu$M SNP increased leaf chlorophyll levels of Gisela 6. SNP augmented leaf carbohydrate and proline content, but it diminished root carbohydrate content. In BA + SNP or NAA + SNP treatments, changes in biochemical constituents were dependent on SNP concentrations and genotypes.
Keywords : Auxin, carbohydrate level, chlorophyll content, cytokinin, endogenous proline concentration, nitric oxide, plant tissue culture, rhizogenesis, shoot proliferation