An improved PVS2 cryopreservation technique for Ascocenda Wangsa Gold orchid using protocorm-like bodies

Authors : Arulvilee RAJASEGAR, Asyraf MANSOR, Ranjetta POOBATHY, Elayabalan SIVALINGAM, Uma Rani SINNIAH, SREERAMANAN SUBRAMANIAM

Pages : 202-0

Doi:10.3906/biy-1405-7

View : 17 | Download : 13

Publication Date : 2015-12-01

Article Type : Research Paper

Abstract :Ascocenda Wangsa Gold is a new and fascinating orchid hybrid in the Malaysian flower industry. An efficient plant vitrification solution 2 insert ignore into journalissuearticles values(PVS2); cryopreservation technique was developed for protocorm-like bodies insert ignore into journalissuearticles values(PLBs); of Ascocenda Wangsa Gold orchid. Parameters assessed included the effect of PVS2 exposure periods, thawing duration, temperature, and culture conditions based on 2,3,5-triphenyltetrazolium chloride absorbance readings and regrowth rates. A regrowth rate of 33.3% was obtained after 2 months when the PLBs were dehydrated in PVS2 for 30 min. The growth rate was improved to 47% when thawing was conducted at 45 °C for 85 s. The highest growth rate insert ignore into journalissuearticles values(53.3%); was obtained when the PLBs were subjected to a 7-day dark treatment before being transferred to a 16-h/8-h light/dark photoperiod. Histological analyses were conducted to study the morphology of cryopreserved and noncryopreserved PLBs of Ascocenda Wangsa Gold. The vitrification protocol developed in this study is a feasible and safe method for strengthening the germplasm conservation of this orchid for commercial purposes.
Keywords : Plant vitrification solution 2, liquid nitrogen, 2, 3, 5 triphenyltetrazolium chloride assay, Ascocenda Wangsa Gold, histology